%0 Journal Article
%T Determination of Monensin Residue in Chicken by HPLC with Post Column Derivatization<br>柱后衍生高效液相色谱法测定鸡肉中莫能菌素残留量
%A Chen Xiaomei
%A Shi Xuxia
%A <br>陈笑梅
%A 施旭霞
%J 色谱
%D 1999
%I 
%X The monensin residue was extracted from the tissue by homogenization with methanol-water and the extract was filtered and partitioned with dichloromethane. The dichloromethane extract is concentrated and clean up by passing through a silica gel cartridge. The analyte on the cartridge is then eluted with dichloromethane-methanol. The eluate is collected and evaporated to dryness. The residue is dissolved and made to a definite volume with 1 mL methanol and the solution is used for post-column derivatization-HPLC determination. Monensin is separated on mu-Bondapak C18 column (3.9 mm i.d. x 300 mm) with methanol-water-phosphoric acid as a mobile phase and the flow rate was 0.7 mL/min. The eluted monensin was reacted with vaniline under acidic and heated condition in post-column derivatization system then detected at 520 nm and quantitated by external standard method. The derivatization reagent consisted of 20 mL concentrated sulfuric acid, 950 mL methanol and 30 g vaniline. The flow rate was 0.7 mL/min. The reactor was a stainless steel coil (300 cm x 1 mm i.d.) set in a 90 degrees C oven. The response values was linear between 20-200 ng. The recovery was 88.1%-101.3%. The coefficient of variation was 0.1%-0.73%.
%K high performance liquid chromatography
%K post column derivatization
%K monensin
%K poultry<br>高效液相色谱法，柱后衍生，莫能菌素，禽肉
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862A035384F15713176&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=CA4FD0336C81A37A&sid=E44E40A2398D4F2A&eid=9C65ADEB5990B252&journal_id=1000-8713&journal_name=色谱&referenced_num=6&reference_num=0