%0 Journal Article
%T Analysis of Headspace Constituents of Gardenia Flower by GC/MS with Solid-Phase Microextraction and Dynamic Headspace Sampling<br>固相微萃取-气相色谱/质谱分析栀子花的头香成分
%A LIU Bai-zhan
%A GAO Yun
%A <br>刘百战
%A 高芸
%J 色谱
%D 2000
%I 
%X The headspace constituents of fresh Gardenia flower were investigated by GC/MS. The headspace volatiles were sampled by solid-phase microextraction (SPME) and dynamic headspace sampling (DHS). SPME sampling was conducted with 100 microns PDMS fiber at 28 degrees C for 60 min. In DHS sampling, purified nitrogen was used as purging gas with a flow rate at 80 mL/min for 120 min. Tenax GR(20 mesh-40 mesh) was used as adsorbent and the volatiles were eluted by ether, and concentrated to 0.5 mL for GC/MS analysis. A Supelco-wax capillary column (30 m x 0.25 mm i.d. x 0.25 micron df) was employed in GC/MS analysis. Initial oven temperature was kept at 45 degrees C for 2 min, then raised to 250 degrees C at 4 degrees C/min, and kept at 250 degrees C for 10 min. According to SPME-GC/MS analysis, the main compounds in headspace of fresh Gardenia flower included farnesene(64.86%), cis-ocimene(29.33%), linalool(2.74%), cis-3-hexenyl tiglate(1.34%), methyl benzoate(0.25%). Results obtained from SPME and DHS sampling were also compared. In this study, SPME afforded a simpler and more sensitive sampling method, and much more accurate information about headspace volatiles of Gardenia flower.
%K gas chromatography/mass spectrometry
%K Gardenia
%K flower
%K headspace volatile compound
%K solid
%K phase microextraction
%K dynamic headspace<br>栀子花
%K 头香化合物
%K 植物香料
%K 分析
%K GC／MS
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=7DCCC32AE46FFAC7&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=94C357A881DFC066&sid=30F3EEEA29E34EE7&eid=366B1248A15658C5&journal_id=1000-8713&journal_name=色谱&referenced_num=25&reference_num=1