%0 Journal Article
%T Analysis of 8-oxo-7,8-dihydro-2''-deoxyguanosine using ultra high performance liquid chromatography-tandem mass spectrometry<br>超高效液相色谱-串联质谱法测定氧化损伤标志物8-羟基脱氧鸟苷
%A SONG Yuling
%A WANG Hailin
%A <br>宋玉玲
%A 汪海林
%J 色谱
%D 2010
%I 
%X An ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the analysis of biomarker 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-OHdG) in deoxyribonucleic acid (DNA) was developed. The artificial oxidation of 2′-deoxyguanosine (dG) at numerous stages in the sample preparation bring a challenge to the accurate measurement of 8-OHdG in DNA. To avoid the artificial oxidation during the enzymatic digestion, desferrioxamine mesylate as a protectant was added into the mixtures. By utilizing YM-3 Centricon membrane (3000 of relative molecular mass cut off), excess proteins in enzymatic solutions were effectively removed, allowing direct UPLC-MS/MS analysis. The UPLC-MS/MS analysis showed a linear relationship between the peak areas and the amounts of 8-OHdG in the range of 17.6~1400 fmol, and the correlation coefficient was 0.9998. By using the developed method, the content of 8-OHdG in calf thymus DNA (CT DNA) was estimated about 12.9±2.35 (calculated as 8-OHdG/106 dG), which was consistent with the previous work. This method can also be applicable for the detection of 8-OHdG in DNA under various oxidative stresses.
%K ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
%K 8-oxo-7
%K 8-dihydro-2`-deoxyguanosine (8-oxodGuo)
%K 2`-deoxyguanosine (dGuo)
%K calf thymus DNA (CT DNA)<br>超高效液相色谱-串联质谱
%K 8-羟基脱氧鸟苷
%K 脱氧鸟苷
%K 脱氧核糖核酸
%K 小牛胸腺
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=049C21FEC8FBD8DEE042A2434BDE7DAA&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=59906B3B2830C2C5&sid=D60A23E3DA48A028&eid=FB318F788ECEF1B2&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=11