%0 Journal Article
%T Preparative isolation and purification of the active components from Viticis Fructus by high-speed counter-current chromatography<br>高速逆流色谱分离纯化蔓荆子中的活性成分
%A GUAN Renjun
%A WANG Daijie
%A YU Zongyuan
%A WANG Xiao
%A LAN Tianfeng
%A <br>管仁军
%A 王岱杰
%A 于宗渊
%A 王晓
%A 蓝天凤
%J 色谱
%D 2010
%I 
%X Vitex trifolia L. var. simplicifolia Cham. is widely distributed in Asia, and its fruits are used as a folk medicine for headaches, colds, migraine, eyepain, etc. In order to effectively separate high-purity active components from the seeds of Vitex trifolia L. var. simplicifolia Cham., a high-speed counter-current chromatography (HSCCC) procedure was performed to separate four components from the crude extract of the fruits. A two-phase solvent system composed of light petroleum-ethyl acetate-methanol-water (3:6:3.6:3, v/v/v/v) was used. Within 230 min, 23 mg of 4-hydroxybenzoic acid, 15 mg of 3,6,7-trimethylquercetagetin, 24 mg of casticin and 5 mg of artemetin were obtained from 250 mg of the crude extract of Viticis Fructus in one-step elution under the conditions of a flow rate of 1.5 mL/min, 800 r/min and the detection wavelength of 254 nm. The purities of the four fractions were 93.1%, 97.3%, 98.7% and 98.5%, respectively. The obtained fractions were analyzed by high performance liquid chromatography (HPLC), and identified by electrospray ionization mass spectrometry (ESI-MS),1H-nuclear magnetic resonance (NMR) and 13C-NMR. The results indicate that HSCCC is a powerful technique for the purification of active components from Viticis Fructus.
%K high-speed counter-current chromatography (HSCCC)
%K 4-hydroxybenzoic acid
%K 3
%K 6
%K 7-trimethylquercetagetin
%K casticin
%K artemetin
%K Viticis Fructus<br>高速逆流色谱
%K 对羟基苯甲酸
%K 3
%K 6
%K 7-三甲基槲皮万寿菊素
%K 蔓荆子黄素
%K 蒿黄素
%K 蔓荆子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=976E428081443E11713CBE0BDC3414E1&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=708DD6B15D2464E8&sid=42AB3C691163F5B1&eid=1EA033F7E0CC5F25&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=0