%0 Journal Article
%T Determination of Lactitol in Mice Serum by High Performance Liquid Chromatography<br>对小鼠血清中乳梨醇含量的测定
%A Ni Weifang
%A Zhang Minbo
%A Huang Zongyu
%A <br>倪维芳
%A 张敏波
%A 黄宗玉
%J 色谱
%D 1998
%I 
%X An HPLC method for the determination of lactitol in mice serum was developed. A liquid chromatograph equipped with a refractive index detector and a Waters Sugar-Pakl column was used. The column temperature was maintained at 90 degrees C and the mobile phase was redistilled water. One milliliter of the serum sample was treated with 3.0 mL methanol. The precipitate was centrifuged for 15 min at 10,000 r/min. Two milliliters of the supernatant was dried on a water bath at 100 degrees C. The residue was dissolved with 1.0 mL water and 10 microL of which was injected. The average recovery was 98.6%. The detection limit was 0.1 microgram (S/N = 3.75). The linear range was 0-600 mg/L (r = 0.9992). Both the RSD's within day and between days were below 1.0% (n = 5).
%K high performance liquid chromatography
%K refractive index detector
%K mice serum
%K lactitol<br>示差折光
%K 小鼠血清
%K 乳梨醇
%K 血药浓度
%K 测定
%K HPLC
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63DD3C09A160E6CCA22&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=B31275AF3241DB2D&sid=D45762219109E903&eid=A726E84831FE609B&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=0