%0 Journal Article
%T Separation of Catechins and Caffeine in Tea Polyphenol byIsocratic Elution High Performance Liquid Chromatography<br>等度反相高效液相色谱法测定茶多酚中的儿茶素和咖啡因
%A TANG Gen yuan
%A WU Hong jing
%A WU Ling
%A LI Zhao ji
%A YAO Yuan gen
%A <br>唐根源
%A 吴红京
%A 吴棱
%A 李兆基
%A 姚元根
%J 色谱
%D 2001
%I 
%X The catechins, particularly in green tea, have been found to possess anti-mutagenic and anti-tumorigenic properties. As each catechin possesses distinct properties, a simple and rapid method that could be used for analysis of individual catechins in a complex mixture would be necessary. A relatively simple and rapid method for simultaneous separation of five catechins and caffeine in tea polyphenol by isocratic elution high performance liquid chromatography has been developed. The analysis could be finished within 30 min. They were measured using Resolve C18 column (at 43 degrees C) and UV detector (at 280 nm), water-85% phosphoric acid aqueous solution-acetonitrile-dimethyl formamide(DMF) (859:1:120:20, V/V) as mobile phase. There was a good linear relationship between the content of component and its peak area for catechins and caffeine, with the correlation coefficients of 0.9992-0.9999. The average recoveries (n = 5) were 83.33%-104.42%, and the relative standard deviations (n = 6) were 0.74%-1.43%. The effect of concentration of DMF in mobile phase was studied.
%K isocratic elution high performance liquid chromatography
%K catechin
%K caffeine
%K tea polyphenol<br>等度高效液相色谱法
%K 儿茶素
%K 咖啡因
%K 茶多酚
%K 测定
%K 茶叶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=FC58A1B92110862C&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=38B194292C032A66&sid=FD7C952458BFB5D8&eid=FBCA02DBD05BD4EA&journal_id=1000-8713&journal_name=色谱&referenced_num=14&reference_num=6