%0 Journal Article
%T Analysis of Trehalose in Transgenic Tobacco Extracts by High Performance Liquid Chromatography with Evaporative Light Scattering Detection<br>液相色谱/蒸发光散射测定转基因烟草提取液中的海藻糖
%A WEI Yang
%A DING Ming yu
%A <br>魏泱
%A 丁明玉
%J 色谱
%D 2001
%I 
%X A high performance liquid chromatographic method with ethylenediamine dynamically modified silica column and evaporative light-scattering detection (ELSD) was developed for the direct determination of trehalose in transgenic tobacco extracts. The separation column (Zorbax Rx-SIL, 4.6 mm i.d. x 250 mm, 5 microns, Hewlett-Packard, USA) modified by ethylenediamine and a guard column (Zorbax Rx-SIL, 4.6 mm i.d. x 12.5 mm, 5 microns) were used. The mobile phase was a mixture of acetonitrile-water(2.6:1, V/V) containing 0.03% ethylenediamine. Regression equations revealed the linear relationships (r = 0.996-0.999) between the concentrations and peak areas of carbohydrates detected by ELSD. The detection limits of ELSD (S/N = 3:1) were 10 mg/L, 20 mg/L, 10 mg/L and 10 mg/L for fructose, glucose, sucrose and trehalose, respectively.
%K high performance liquid chromatography
%K ethylenediamine dynamically modified silica column
%K evaporative light scattering detection
%K trehalose<br>高效液相色谱法
%K 乙二胺动态修饰硅胶柱
%K 蒸发光散射检测
%K 测定
%K 转基因
%K 烟草提取液
%K 抗性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=581E9A99DC47D04F&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=38B194292C032A66&sid=6ED15D8DCB279BC4&eid=F8035C8B7D8A4264&journal_id=1000-8713&journal_name=色谱&referenced_num=6&reference_num=9