%0 Journal Article
%T The Preparation of Chemical-Bonded Hyperbranched Polymer Coated Column for Capillary Electrophoresis and Their Application to Separation of Basic Proteins<br>超支化聚合物化学键合毛细管电泳柱的制备及其对蛋白质的分离行为
%A SHOU Chongqi~
%A <br>寿崇琦
%A 张志良
%A 周长利
%A 赵春宾
%A 李关宾
%A 陈立仁
%J 色谱
%D 2004
%I 
%X A series of hyperbranched poly(amine-ester)s were synthesized using one-step and pseudo-step procedure respectively, and their molecular structures were characterized by IR, hydroxyl value and viscosity. These polymers were coated on the inner surface of the fused-silica capillaries in a chemical bonding process. The electroosmotic flow and the separation capability for basic proteins of the coated capillaries were investigated. The results demonstrated that the coating in the capillaries could greatly reduce the electroosmotic flow and effectively suppress protein adsorption in the pH range of 3-7. The separation efficiency of the coated capillary of hyperbranched poly(amine-ester)s with trimethylolpropane and pentaerythritol as the core was 10~5/m and 10~7/m, respectively. The separation efficiency and the stability of basic proteins on these coated capillaries were found excellent.
%K capillary electrophoresis
%K protein
%K polymer coated column
%K hyperbranched polymer
%K chemically modified silica surface
%K electroosmotic flow<br>毛细管电泳
%K 蛋白质
%K 涂层柱
%K 超支化聚合物
%K 化学修饰硅胶表面
%K 电渗流
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=2AC9DAC472946921&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=38B194292C032A66&sid=9F8C5EF901EA1E7E&eid=44A4891E33BFF455&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=7