%0 Journal Article
%T Determination of cyanuric acid in milk and milk powder by ultra performance liquid chromatography-tandem mass spectrometry with post-column ammoniation<br>柱后氨化-超高效液相色谱-串联质谱法测定牛奶和奶粉中的三聚氰酸
%A YUN Huan
%A ZHANG Zhaohui
%A GAO Yangyang
%A HE Yue
%A <br>云环
%A 张朝晖
%A 高洋洋
%A 何悦
%J 色谱
%D 2010
%I 
%X A method for the determination of cyanuric acid in milk and milk powder has been developed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with post-column ammoniation. After extracted by 5% trichloroacetic acid, the samples were loaded onto an Acquity UPLC HSS T3 column (50 mm×2.1 mm, 1.8 μm) and separated with a gradient elution. The electrospray was operated in the negative mode and monitored by the multiple reaction monitoring (MRM) mode. The calibration curves showed a good linearity in the range of 10.0~160.0 μg/L, and the correlation coefficients (r) were higher than 0.99. When the spiked levels were 0.05, 0.10, and 0.20 mg/kg, the recoveries of cyanuric acid in milk ranged from 60% to 118.5%, with the relative standard deviations (RSDs) of 8.2%~12.6%; while the spiked levels were 0.25, 0.50, and 1.00 mg/kg in the milk powder, the recoveries ranged from 88% to 108% and the RSDs ranged from 2.5%~5.7%. The limits of quantification (LOQs, S/N=10) of cyanuric acid were 0.05 mg/kg in milk sample and 0.25 mg/kg in milk powder. The results indicate that the method is simple, rapid, sensitive and suitable for the qualitative and quantitative analyses of cyanuric acid in milk and milk powder samples.
%K post-column ammoniation
%K ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
%K cyanuric acid
%K milk
%K milk powder<br>柱后氨化
%K 超高效液相色谱-串联质谱
%K 三聚氰酸
%K 牛奶
%K 奶粉
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=5C0B6CD617319F3E17F526938D65ED20&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=708DD6B15D2464E8&sid=8E4B4A74C5BF6530&eid=0342E221E01D5238&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=0