%0 Journal Article
%T Isolation and Purification of the Active Principle from Agkistrodon Acutus Snake Venom by High Performance Ion Exchange Liquid Chromatography<br>高效离子交换色谱法分离皖南尖吻蝮蛇毒活性组分
%A Y Qian
%A X Shou
%A X Cheng
%A C Wang
%A <br>钱亚雯
%A 寿晓海
%A 承新
%A 王淳
%J 色谱
%D 1998
%I 
%X The active component from Agkistrodon acutus snake venom was further purified by high performance ion-exchange liquid chromatography over a Protein-PAK DEAE-8HR column(10.0 mm i.d. x 100 mm). The elution was operated with a linear gradient from 10% to 100% NaCl-0.02 mol/L Tris-HCl buffer(pH 8.0). The quantitative results were calculated as 45.41% based on peak area nomalization and the purity of the active component was 90% determined by electrophoresis.
%K high performance ion exchange liquid chromatography
%K Agkistrodon acutus snake venom
%K active component<br>皖南
%K 尖吻蝮蛇毒
%K 活性组分
%K 蝮蛇毒
%K 蛇毒
%K 分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63DCD12A665FA021C4A&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=38B194292C032A66&sid=4D7D059FFBF006B9&eid=30897FA31CA3354D&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=4