%0 Journal Article
%T Preparation of Senkyunolide Ⅰ by Reversed-Phase High Performance Liquid Chromatography<br>反相高效液相色谱法制备洋川芎内酯Ⅰ
%A ZHANG Xiaozhe
%A XU Qing
%A XIAO Hongbin
%A LIANG Xinmiao
%A <br>张晓哲
%A 徐青
%A 肖红斌
%A 梁鑫淼
%J 色谱
%D 2004
%I 
%X A method for the isolation of senkyunolide I from Chuanxiong with reversed-phase preparative high performance liquid chromatography (HPLC) is described. After 95% ethanol extract was obtained with solvent extraction and normal-phase silica column separation, a fraction containing senkyunolide I was acquired and subjected to preparative HPLC for the isolation of target component. In the experiment a solvent system consisting of methanol-0.05 mol/L NH4Ac aqueous solution was found effective to eliminate the interference of ferulic acid that was the major compound in the sample. Senkyunolide I and ferulic acid were separated completely by using a stepwise gradient solvent system of 10% methanol in 10 min, and 60% methanol in 10 - 40 min. The subfractions were collected and subjected to an open MCI gel CHP-20P column for desalination respectively. Senkyunolide I was obtained and the structure was identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR). This method is effective and reliable for the preparation of senkyunolide I from Chuanxiong.
%K NULL<br>洋川芎内酯Ⅰ
%K 制备工艺
%K 反相高效液相色谱法
%K 阿魏酸
%K 川芎
%K 分离
%K 纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=1B7AB242A60A800A&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=CA4FD0336C81A37A&sid=2001E0D53B7B80EC&eid=BE33CC7147FEFCA4&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=8