%0 Journal Article
%T Determination of Catechol-O-Methyltransferase Activity in Human Erythrocytes by High Performance Liquid Chromatography<br>高效液相色谱法测定人体红细胞中儿茶酚氧位甲基转移酶的活性
%A YAN Xiao hua
%A DENG Xiao min
%A WU Huai an
%A <br>闫小华
%A 邓小敏
%A 吴怀安
%J 色谱
%D 2001
%I 
%X A rapid assay method for catechol-O-methyltransferase (COMT) activity in human erythrocyts by high performance liquid chromatography (HPLC) with UV detection was established. Enzyme activity was determined from erythrocyte lysates using S-adenosyl-L-methionine (SAM) as methyl donor and 3, 4-dihydroxybenzoic acid (DBA) as substrate. The 3-O-methylated reaction products were measured by HPLC with UV detection. The linear range of COMT was from 1 U/mL to 60 U/mL with an average RSD < 10%, and the detection limit was 0.5 U/mL(S/N > or = 5).
%K high performance liquid chromatography
%K catechol
%K O
%K methyltransferase
%K activity
%K erythrocyte<br>高效液相色谱法
%K 儿茶酚氧位甲基转移酶
%K 活性
%K 红细胞
%K 测定
%K 人
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=914D01E9AB7E787D&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=38B194292C032A66&sid=D6354F61445E9456&eid=6CCE24D86D03D083&journal_id=1000-8713&journal_name=色谱&referenced_num=6&reference_num=6