%0 Journal Article
%T Preparative isolation and purification of ergosterol from a strain of Paecilomyces hepialid by high-speed counter-current chromatography<br>高速逆流色谱法从蝙蝠蛾拟青霉中快速分离制备麦角甾醇纯品
%A ZHANG Nengsheng
%A WANG Jinbin
%A WANG Xiaoyan
%A WANG Xiaodong
%A HU Fenglin
%A <br>章能胜
%A 王金彬
%A 汪小艳
%A 王小董
%A 胡丰林
%J 色谱
%D 2010
%I 
%X To develop an effective and rapid method for the preparation of ergosterol from a strain of Paecilomyces hepialid, the ethyl acetate extract of the mycelia was injected into a high-speed counter-current chromatograph (HSCCC) directly, and eluted with different solvent systems. The result showed that the solvent system composed of n-hexane-ethyl acetate-methanol-water (6:1.7:6:0.3, v/v/v/v) was the best. The lower phase was used as the mobile phase and performed at a flow rate of 2 mL/min, while the apparatus rotated at 850 r/min, and detected at 280 nm. The prepared ergosterol was identified with ultraviolet detection (UV), high resolution mass spectrometer (HRMS) and standard, and its purity was 99.2% analyzed by high performance liquid chromatography. The established method is relatively simple, fast, and suitable for the large-scale isolation and separation of ergosterol.
%K high-speed counter-current chromatography (HSCCC)
%K preparation
%K ergosterol
%K Paecilomyces hepialid<br>高速逆流色谱
%K 制备
%K 麦角甾醇
%K 蝙蝠蛾拟青霉
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=7633DD588C7C9E394713E069BB0BB65E&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=CA4FD0336C81A37A&sid=68D88C2FCF9C3098&eid=AA76E167F386B6B3&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=15