%0 Journal Article
%T Electrophoretic Migration Behavior of Deoxyribonucleic Acid Fragments in Three Polymer Solution Concentration Regions<br>脱氧核糖核酸分子在高分子溶液3个不同浓度区间的电泳迁移行为
%A JIN Yan
%A LIN Bing cheng
%A FENG Ying sheng
%A <br>靳艳
%A 林炳承
%A 冯应升
%J 色谱
%D 2001
%I 
%X The theory of polymer coils shrinking in semi-dilute solution has recently been developed on polymer solution. The polymer solution from coils shrinking concentration Cs to uniform entangled concentt concentration C+ has been defined as semi-dilute solution. We experimentally investigated the electrophoretic migration behavior of 100 bp deoxyribonucleic acid (DNA) Ladder in hydroxypropyl methyl cellulose (HPMC) concentration ranging from 1.25 g/L to 16.06 g/L. The friction force mobility mu f is used to express the friction force that DNA will encounter in capillary electrophoresis. Our results indicate the division of polymer solution into three regions depending on the relationship of mu f and HPMC concentration and Ferguson plot. Resolutions of 200 bp/300 bp and 700 bp/800 bp show semi-dilute polymer solution suits large fragments and small fragments DNA separation respectively. the results confirm that the current polymer theory is valid under actual CE condition.
%K capillary electrophoresis
%K deoxyribonucleic acid
%K theory of coil shrinking in semi
%K dilute polymer solution<br>毛细管电泳
%K 脱氧核糖核酸
%K 高分子亚浓溶液线团收缩理论
%K 高分子溶液
%K 电泳迁移
%K DNA
%K 分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=9FB964B13D92CF7F&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=CA4FD0336C81A37A&sid=BFE7933E5EEA150D&eid=E84BBBDDD74F497C&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=8