%0 Journal Article
%T Separation of Enantiomeric Labetalol by Reversed-Phase High Performance Liquid Chromatography<br>柱前手性衍生化反相高效液相色谱法分离拉贝洛尔对映体
%A Zhao Hua
%A Li Huizhi
%A Qiu Zongyin
%A <br>赵华
%A 李惠芝
%A 邱宗荫
%J 色谱
%D 1999
%I 
%X A reversed-phase high performance liquid chromatographic method for the separation of labetalol enantiomers was developed. In the method, 2, 3, 4, 6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate (GITC) was chosen as the reagent for pre-column chiral derivatization of labetalol to give four diastereomeric thiourea derivatives. These derivatives were efficiently separated on a Nova-Pak C18 column using V(MeOH):V(0.01 mol.L-1(pH 7.00) H2PO4(-)-HPO(4)2- buffer) = 51:49 as the mobile phase and detected by UV detector at a wavelength of 250 nm or fluorescence detector at lambda ex = 340 nm and lambda em = 440 nm. The effects of the pH of mobile phase on the retention and fluorescence absorbance are also discussed.
%K NULL<br>HPLC
%K 对映体
%K 拆分
%K 拉贝洛尔
%K 光学异构体
%K GITC
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862627F1E234E459C16&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=E158A972A605785F&sid=F7BB24011DC0D223&eid=23410D0BDB501DF5&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=5