%0 Journal Article
%T Simultaneous Determination of Cd2+, Pb2+, Cu2+ and Zn2+ by Reversed-Phase High Performance Liquid Chromatography<br>反相高效液相色谱法同时测定镉、铅、铜和锌
%A YIN Jiang-wei
%A WANG Guang-jian
%A XIAO Zhi-fang
%A <br>尹江伟
%A 王光建
%A 肖志芳
%J 色谱
%D 2000
%I 
%X HPLC was used in the investigation of the reaction behavior of Cd2+, Pb2+, Cu2+ and Zn2+ with mesotetrakis (4-hydroxy-phenyl)porphine (THPP) and chromatographic behavior of their complexes. The reaction and chromatographic separation parameters were optimized and a method for the simultaneous determination of Cd2+, Pb2+, Cu2+ and Zn2+ by reversed-phase high performance liquid chromatography with THPP as pre-column derivatization reagent was established. The metal-THPP complexes formed in 0.1 mol/L borate buffer (pH 10.5) by heating in 60 degrees C water bath for 20 min and were extracted into ethyl acetate-benzene mixture. The metal-THPP complexes and THPP were separated on a Prodigy ODS, 5 microns, 250 mm x 4.6 mm i.d. column with methanol-0.05 mol/L borate buffer(90:10, V/V) (pH 8.0) as eluent and detected at 437 nm (Cd2+ and Zn2+), 465 nm (Pb2+) and 416 nm (Cu2+). The detection limits of the four metal ions in 5 microL injection solution were Cd2+ 0.02 ng, Pb2+ 0.02 ng, Cu2+ 0.02 ng, and Zn2+ 0.12 ng, and the linear ranges were Cd2+ 8 ng-1.5 micrograms, Pb2+ 8 ng-3.0 micrograms, Cu2+ 8 ng-5.0 micrograms, and Zn2+ 50 ng-10 micrograms in 10 mL reaction solution. The intra-day repeatability (as RSDs) was in the range of 2.8%-4.8% and the inter-day reproducibility was in the range of 3.7%-9.7%.
%K reversed
%K phase high performance liquid chromatography
%K cadmium
%K lead
%K copper
%K zinc<br>反相高效液相色谱法
%K 镉
%K 铅
%K 铜
%K 锌
%K 同时测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=491BB2CE7FD7D33D&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=94C357A881DFC066&sid=08076B8B3CC96095&eid=BEE722AB5028E81F&journal_id=1000-8713&journal_name=色谱&referenced_num=12&reference_num=4