%0 Journal Article
%T Determination of 10-HDA in Honeybee Body by HPLC<br>高效液相色谱法测定蜜蜂蜂体王浆酸
%A Fan Hui
%A He Chengshun
%A Han Hongyan
%A <br>范晖
%A 何承顺
%A 韩红岩
%J 色谱
%D 1999
%I 
%X In the present work we found that in the honeybee body there exists an unsaturated fatty acid, trans-10-hydroxy-2-decenoic acid (10-HDA), which was known only to be present in royal jelly. We established the analytical method of 10-HDA in honeybee body by HPLC and simplified the extraction method of 10-HDA. In the optimum conditions the linear range of detection was 10-1,000 ng, the correlation coefficient was 0.9998, the recovery was 96.5%-99.2% and the detectable limit was 0.53 microgram/g.
%K ?high performance liquid chromatography
%K honeybee body
%K 10-HDA<br>蜜蜂
%K 蜂体
%K 王浆酸
%K HPLC
%K 测定
%K HDA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862A2012FA14AB57EFC&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=38B194292C032A66&sid=DFEE4E8C33C95CEF&eid=119B6C0AA09DE6B9&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=5