%0 Journal Article
%T Simultaneous Determination of Amino Acids in Food by Capillary Electrophoresis with Indirect Ultraviolet Detection<br>高效毛细管电泳间接紫外吸收检测法测定食品中的氨基酸
%A CHEN Bing
%A LI Xiaoge
%A HE Ping
%A XIANG Xiaolan
%A <br>陈冰
%A 李小戈
%A 何萍
%A 项小兰
%J 色谱
%D 2004
%I 
%X The characteristic of capillary electrophoresis (CE) with indirect ultraviolet (UV) detection of L-ornithine (Orn), L-proline (Pro) and L-glutamine (Gln) was investigated. A suitable electrolyte consists of 5 mmol/L sodium p-aminobenzene sulfonic acid-10mmol/L KH_2PO_4 ( pH 11.5) with UV detection at 254 nm. The applied voltage was set at 12 kV. The three amino acids were completely separated and detected by the method in 11 min. The relative standard deviations (RSD) of the method were better than 0.72% for migration time and 2.0% for peak height. The detection limits of Orn, Pro and Gln were 6.78, 8.71 and 7.86 mg/L, respcetively. The proposed method was successfully applied to the determination of amino acids in foods (Haitian soybean sauce, Tsingtao beer and amino acids tonic) and the recoveries were in the range of 96.8%-104%.
%K high performance capillary electrophoresis
%K indirect ultraviolet detection
%K amino acid
%K Haitian soybean sauce
%K Tsingtao beer
%K amino acids tonic
%K food<br>食品分析
%K 氨基酸
%K 测定
%K 高效毛细管电泳-间接紫外吸收法
%K 海天生抽
%K 青岛啤酒
%K 氨基酸口服液
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A1A2A4951A092206&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=CA4FD0336C81A37A&sid=67969BA850333433&eid=228A710F49B6CE58&journal_id=1000-8713&journal_name=色谱&referenced_num=8&reference_num=8