%0 Journal Article
%T Preparation of Novel Macroporous Silica Based Amide Polymer Bonded Packing and Its Application to the Separation of Proteins<br>新型大孔硅质酰胺型聚合物键合相的制备及其对蛋白质的分离
%A Wei Yun
%A Fan Limin
%A Jiang Shengxiang
%A Chen Liren
%A <br>魏芸
%A 樊立民
%A 蒋生祥
%A 陈立仁
%J 色谱
%D 1998
%I 
%X A novel macroporous silica based amide polymer bonded packing for protein separations in HPLC is described.The macroporous silica support was bonded with diethoxymethyl vinyl silane and then copolymerized with methylacrylamide and divinylbenzene to produce a tailored stationary phase with high resolution and inertness.The repeatability of packing preparation is good.They were characterized through the application of a standard protein mixture of pepsin,glucose oxidase,bean trypsinogen inhibitor and ribonuclease.The time for elution of these proteins is less than 12 minutes.It is suggested that the macroporous silica based amide polymer bonded packing can be used to quickly separate biopolymer.This packing is a better alternative for the biopolymer separation.
%K stationary phase
%K macroporous silica
%K amide polymer bonded phase
%K protein separation<br>固定相
%K 分析
%K 蛋白质
%K 分离
%K 色谱柱填料
%K 填料
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63DF929EE9D1FB0877E&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=38B194292C032A66&sid=AC1578C6BB9EBDEF&eid=5D9D6A8FC2C66FD8&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=4