%0 Journal Article
%T Separation and Determination of Eight Plant Hormones by Reversed Phase High Performance Liquid Chromatography<br>植物激素的反相高效液相色谱法分离和测定
%A Fang Nenghu
%A Hou Shuquan
%A Shao Xueguang
%A He Youzhao
%A Zhao Guiwen
%A <br>方能虎
%A 侯树泉
%A 邵学广
%A 何友昭
%A 赵贵文
%J 色谱
%D 1998
%I 
%X In this paper, reversed-phase high performance liquid chromatographic technique was used for the separation and determination of eight plant hormones. Methanol-water-acetic acid system was chosen as the mobile phase. The effects of different separation conditions, such as the methanol and acetic acid concentrations in mobile phase, on the retention behaviours of eight plant hormones in this system were studied. The general trends in retention behaviours could be correlated to the methanol concentration in mobile phase. The experimental results showed that the optimum separation was achieved with following gradient elution condition: 0-3 minutes, 70% (water percentage in mobile phase), 3-13 minutes, 70%-20%, 13-48 minutes, 20%. Benzene was added to be as the internal standard. Under this experimental condition, the eight plant hormones could be separated completely and detected quantitatively at 260 nm within 16 minutes. The calibration curves for the eight compounds gave linearity over a wide range. The correlation coefficients of each components were r(ZT) = 0.9971, r(GAs) = 0.9999, r(K) = 0.9997, r(BA) = 0.9995, r(IAA) = 0.9998, r(IPA) = 0.9982, r(IBA) = 0.9995 and r(NAA) = 0.9995. The method is rapid, simple and efficient. It is a suitable method for the accurate determination of gibberellic acid (GA) and alpha-naphthaleneacetic acid (alpha-NAA) in products for agricultural use.
%K reversed phase high performance liquid chromatography
%K plant hormone
%K separation and detection<br>HPLC
%K 植物激素
%K 分离
%K 分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63D5633A08B8886B108&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=94C357A881DFC066&sid=84A93BA251D28205&eid=5335AD3CFE6E14EA&journal_id=1000-8713&journal_name=色谱&referenced_num=16&reference_num=4