%0 Journal Article
%T Determination of organic acids in honey by solid phase extraction-high performance liquid chromatography<br>固相萃取-高效液相色谱法测定蜂蜜中的有机酸
%A ZHU Xiaoling
%A YE Fei
%A YANG Jie
%A XIAO Xiao
%A WEN Hong
%A LIU Rui
%A <br>朱晓玲
%A 叶飞
%A 杨洁
%A 肖潇
%A 文红
%A 刘睿
%J 色谱
%D 2010
%I 
%X A method for the simultaneous determination of 5 organic acids (L-malic acid, maleic acid, succinic acid, citric acid, D-malic acid) in honey was developed by solid-phase extraction and high performance liquid chromatography (SPE-HPLC). The sample was purified by a Bond Elutes SAX SPE cartridge after pretreatment, then separated using a reversed-phase C18-MS-IIcolumn. A 2% metaphosphoric acid solution was used as the mobile phase at a flow rate of 0.7 mL/min. The detection was performed by a diode array detector at 210 nm. Under these conditions, the linear ranges of the method for L-malic acid was 5.0~500 mg/L, for maleic acid was 0.1~500 mg/L, and for succinic acid, citric acid, D-malic acid were 18.0~500 mg/L. The correlation coefficients of 5 organic acids were more than 0.9967. The average recoveries of 5 organic acids were 86.0%~103.9% with the relative standard deviations of 5.7%~9.8% (n=6). The detection limits ranged from 0.06 to 9.4 mg/kg. The method can be used for determining organic acids in honey samples.
%K solid-phase extraction (SPE)
%K reversed-phase high performance liquid chromatography (RP-HPLC)
%K organic acids
%K honey<br>固相萃取
%K 反相高效液相色谱法
%K 有机酸
%K 蜂蜜
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=7EC27DA0006FCC87197720B1A1D4BE57&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=F3090AE9B60B7ED1&sid=D4A72ABDCEDCE1BD&eid=BFB3B49B74E638B4&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=0