%0 Journal Article
%T Investigation of Association Parameters of Basic Drugs to Human Serum Albumin by Capillary Zone Electrophoresis/Frontal Analysis<br>碱性药物与人血清白蛋白结合参数的毛细管区带电泳/前沿分析
%A Ding Yongsheng
%A Zhu Xiaofeng
%A Lin Bingcheng
%A <br>丁永生
%A 朱晓峰
%A 林炳承
%J 色谱
%D 1999
%I 
%X A CZE/FA method was applied to the study of association parameters of basic drugs, verapamil (VER) and propranolol (PRO), to human serum albumin (HSA) under drug-HSA association equilibria. After the direct hydrodynamic injection of a drug-HSA mixed solution into a coated capillary (effective length, 32 cm; 50 microns i.d.), the basic drug is eluted as a zonal peak with a plateau region under condition of phosphate buffer (pH 7.4, ionic strength = 0.17) at 10 kV running voltage. The unbound concentration of drug can be determined from the plateau peak height with good linear response, r = 0.999 for both VER(n = 6) and PRO(n = 8), respectively. Six VER-HSA solutions (containing 50-175 mumol/L of VER and 450-325 mumol/L of HSA) and eight PRO-HSA solutions (containing 50-400 mumol/L of VER and 450-100 mumol/L of HSA) were investigated. Three analytical methods were used for the present CZE/FA system including Scatchard, Klotz and non-linear regression analyses. The estimated association parameters were as follows: the numbers of HSA binding site n for VER were over 1.21(0.04)-1.30(0.09), binding constant, K, was between 1,690(130) and 1,790(70). Whereas for PRO, n = 0.54(0.04)-0.62(0.03), and K = 1,390(70)-1,690(180). The data deduced from these three analyses agreed well with each other.
%K capillary electrophoresis
%K frontal analysis
%K basic drugs
%K human serum albumin
%K association
%K parameter<br>毛细管区带电泳，前沿分析，碱性药物，人血清白蛋白，结合参数
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862BB6EC7B28418B1CC&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=CA4FD0336C81A37A&sid=9FFCC7AF50CAEBF7&eid=BFE7933E5EEA150D&journal_id=1000-8713&journal_name=色谱&referenced_num=6&reference_num=3