%0 Journal Article
%T Determination of serotonin in rat brain by gas chromatography/mass spectrometry]<br>气相色谱/质谱法测定大鼠脑中5-羟色胺的含量
%A T Liang
%A Y Huang
%A K Zhu
%A <br>梁天天
%A 黄亦佳
%A 朱卡琳
%J 色谱
%D 1998
%I 
%X A GC/MS method for the determination of serotonin (5-hydroxytryptamine, 5-HT) has been developed. 5-HT in homogenized rat brain was acylated and extracted into EtOAc. The acylate was further derivatized with heptafluorbutyl anhydride and formed a spirocyclic pentafluoropropionyl derivative. Subsequent analysis of 5-HT derivative by gas chromatography-electron capture negative ion chemical ionization mass spectrometry was highly specific and sensitive. The assay is linear from 0.5 to 50 micrograms/L with a linear equation of Y = 0.134 8X - 0.07995, r = 0.999. The recovery was 98.2% +/- 3.8% (n = 10) and the limit of detection was 0.5 microgram/L. The reproducibility of 5-HT determination in rat brain was less than 10% (RSD) at both levels of 1.0 microgram/L and 50.0 micrograms/L. The method has been applied to analysis of 5-HT in the dose rat brain successfully.
%K gas chromatography/mass spectrometry
%K serotonin
%K rat brain<br>GC
%K MS
%K 5－羟色胺
%K 大鼠脑组织
%K 羟色胺
%K 测定
%K 分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63D9CA05C71AB5C5B46&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=38B194292C032A66&sid=273ADA1BCEFE8C00&eid=9F8C5EF901EA1E7E&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=0