%0 Journal Article
%T Chiral Two-Phase Synergistic Effect in High Performance Liquid Chromatography<br>高效液相色谱中的手性两相协同作用
%A MENG Lei~
%A <br>孟磊
%A 李方楼
%A 袁黎明
%J 色谱
%D 2004
%I 
%X Cellulose tribenzoate (CTB) and cellulose triphenylcarbamate (CTPC) were synthesized for the coating of high performance liquid chromatographic stationary phases. Elemental analysis and IR spectra indicated that most of the hydroxy groups of the microcrystalline cellulose had been converted into ester groups. The silanized gel was coated with CTB/CTPC by using tetrahydrofuran as solvent. The enantiomeric resolutions of phenylalanine (flow rate: 0.5 mL/min) and tryptophan were carried out with a hexane/2-propanol (9:1, v/v) mixture at 25 degrees C, when beta-cyclodextrin (beta-CD), dimethyl-beta-cyclodextrin (DM-beta-CD) and trimethyl-beta-cyclodextrin (TM-beta-CD) were used as mobile phase additives. The results showed that TM-beta-CD at the concentrations higher than 0.60 mmol/L had an explicit chiral two-phase synergistic effect with chiral stationary phase, but beta-CD and DM-beta-CD at any concentrations and TM-beta-CD at low concentrations had no effect.
%K NULL<br>高效液相色谱
%K 手性固定相
%K 手性流动相添加剂
%K 手性两相协同作用
%K 纤维素衍生物
%K β-环糊精衍生物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=E61DAE9CBC222AA9&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=0B39A22176CE99FB&sid=2F56B21F91C9B05B&eid=B344543C2864D684&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=17