%0 Journal Article
%T Application of peptide retention time in proteome research<br>色谱保留时间在蛋白质组研究中的应用
%A SHAO Chen
%A GAO Youhe
%A <br>邵晨
%A 高友鹤
%J 色谱
%D 2010
%I 
%X Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has been one of the most popular approaches in proteome analysis. As an independent parameter to mass spectrometry information, peptide retention time has been utilized to facilitate protein identification and quantification. In the field of peptide identification, the prediction of the retention time combined with routine tandem mass spectrometry database searching methods could help improve the confidence of identification. The sensitivity of identification could also be improved by matching peaks with both the accurate mass and retention time in multiple aligned LC-MS runs. Meanwhile, because small changes of liquid chromatography conditions lead to variability in retention times unavoidably, retention time alignment is crucial to label-free quantification. Additionally, post-translational modifications (PTM) could be identified by combining retention time shifts and mass deviation information.
%K liquid chromatography-tandem mass spectrometry (LC-MS/MS)
%K retention time prediction
%K retention time alignment
%K peptide identification
%K label-free quantification
%K post-translational modification (PTM)
%K proteomics<br>液相色谱-串联质谱
%K 保留时间预测
%K 保留时间比对
%K 多肽鉴定
%K 非标记定量
%K 翻译后修饰
%K 蛋白质组学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=DB9F513BD61772D5BB717C4168C1E7C1&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=0B39A22176CE99FB&sid=1F199509C0B6C4D6&eid=03A030BB0C519C60&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=48