%0 Journal Article
%T LC/MS Research on the Isomers of Special Melanoma Antigen-Encoding Gene-2 Epitope Peptide Induced by Solvent<br>溶剂诱导黑色素瘤抗原基因-2特定表位多肽异构的LC/MS研究
%A ZHOU Wei
%A WU Yu zhang
%A BIAN Jiang
%A JIA Zheng cai
%A TANG Yan
%A ZOU Li yun
%A <br>周伟
%A 吴玉章
%A 边疆
%A 贾正才
%A 唐艳
%A 邹丽云
%J 色谱
%D 2001
%I 
%X To study the influence of different solvent systems on the isomers of melanoma antigen encoding gene 2 (MAGE 2) epitope peptide synthesized by Marrifield's solid synthesis method, MAGE 2(171 179) epitope peptides were pre treated using ethanol and methanol systems respectively, and then analysed by RP HPLC/MS, with dimethyl sulphoxide (DMSO) as control solvent Results demonstrated that 100% ethanol and 100% methanol solvents could induce the production of isomers of MAGE 2, while 100% DMSO, 50% ethanol and 50% methanol could not These isomeric peptides could be reversed greatly if treated with trifluoroacetic acid (TFA) The results also showed that the analysis would be distorted and the yield of peptides would decrease dramatically if the peptides were pre treated with high concentrations of ethanol and methanol before RP HPLC analysis and preparation Thus it is suggested that it might be better to decrease the concentration of organic phase in the solvent system for the resolvable hydrophobic peptides, acidified ethanol and methanol might also be better for the highly hydrophobic peptides, and DMSO would be a good solvent for hydrophobic peptides analysis provided that it would not influence the resolving power of chromatography
%K liquid chromatography/mass spectrometry
%K melanoma antigen
%K encoding gene
%K 2
%K epitope peptide
%K isomer
%K solvent
%K induced<br>液相色谱／质谱联用
%K 黑色素瘤抗原基因－2
%K 表位多肽
%K 异构体
%K 溶剂诱导
%K 黑色素瘤
%K 免疫治疗
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=CF6FD35681A9B074&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=94C357A881DFC066&sid=78BF76CF5B7CB0F2&eid=FB36B1C076A263FA&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=7