%0 Journal Article
%T Synthesis of fluorescent derivatization reagent 2-(4-carboxyphenyl)-naphthoimidazole and its application in the determination of alcohols by high performance liquid chromatography]<br>荧光衍生化试剂2-(4-羧基苯基)-4,5-萘并咪唑的合成及在HPLC测定醇类中的应用
%A H B Zhou
%A Y Mukai
%A <br>周宏兵
%A 向日良夫
%J 色谱
%D 2000
%I 
%X A new sensitive pre-column fluorescent derivatization method, which employs high performance liquid chromatography(HPLC) with fluorometric detection, for the determination of alcohols is described. The alcohols were transformed into the corresponding fluorescent esters by treating with 2-(4-carboxyphenyl)-naphthoimidazole in dichloromethane in the presence of 4-piperidinopyridine (PIP) and 1-isopropyl-3-(3-dimethylaminopropyl)-1-carbodiimide perchlorate(IDC) at 80 degrees C for 10 min. The resultant mixture was analyzed by HPLC on a reversed phase column(Zorbax Bp C8, 4.6 mm i.d. x 250 mm, 5 microns) with acetonitrile-methanol (volume ratio was 90:10) as mobile phase, and the sample volume was 20 microL. Monitored excitation and emission wavelengths were 345 nm and 485 nm respectively in determination of the alcohol. The calibration curve of cholesterol is linear between 0 and 2.5 g/L with correlation coefficient of 0.9992. The average recovery was 96.15% with RSD of 1.39%. The lowest detectable mass concentration of cholesterol was 1.0 microgram/L (S/N = 3).
%K NULL<br>高效液相色谱法
%K 萘并咪唑
%K 醇类测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=CACFBE01AAE1E68BD6DA269746FBB479&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&sid=F3090AE9B60B7ED1&eid=FC0714F8D2EB605D&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=2