%0 Journal Article
%T Preparation and Evaluation of New Ion\|Exchange Chromatographic Stationary Phase for the Use in High Performance Liquid Chromatography<br>新型离子交换硅胶键合相的制备及评价
%A Lei Xiaoling
%A Wang Junde
%A Cong Runzi
%A <br>雷晓玲
%A 王俊德
%A 丛润滋
%J 色谱
%D 1999
%I 
%X A new method for the bonding of diethylamine(DEA) on the surface of silica to prepare novel hydrophilic packings for HPLC has been studied. After allyl glycidyl ether being synthesized, the Si-DEA anion-exchange bonded phase was prepared by the reaction of the double bond in allyl group with Si-H silica. The bonded phases obtained were characterized by elemental analysis, diffuse reflectance infrared Fourier transform(DRIFT) spectroscopy and HPLC evaluation. The methods were used for both porous silica and monodisperse non-porous silica. The contents of carbon, hydrogen and nitrogen of porous Si-DEA packing (MPS-DEA) were 3.31%, 0.95% and 1.34% respectively and those of monodisperse non-porous Si-DEA packing (NPS-DEA) were 2.55%, 0.97% and 0.96% respectively. The diethylamine absorption peak can be observed at 2970 cm-1 from the Si-DEA silica DRIFT spectrum. These data revealed that the diethylamine had been bonded on MPS-DEA and NPS-DEA packings. In HPLC tests, nucleotides and nucleosides such as cytosine, uracil, cytidine-5'-monophosphate, adenosine-5'-monophosphate, inosine-5'-monophosphate and guanosine-5'-monophosphate were satisfactorily separated on the porous anion-exchange packing (MPS-DEA), and a group of proteins (lysozyme, ribonuclease, ovalbumin, bovine serum albumin, insulin and gamma-globulin) were separated within 15 minutes successfuly. All test results indicated that the new method for preparing better anion-exchange silica packings is effective for both porous silica and monodiperse non-porous silica.
%K high performance liquid chromatography
%K stationary phase
%K ion\|exchange chromatography
%K biopolymer separation<br>高效液相色谱
%K 键合固定相
%K 离子交换色谱
%K 硅胶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B86219F841F7B9959999&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=94C357A881DFC066&sid=6D6BFCF0101BC091&eid=E57FE519484CFB70&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=1