%0 Journal Article
%T Determination of HIgG in Human plasma by high performance membrane affinity chromatography(HPMAC)]<br>蛋白A高效亲和膜色谱法测定人血浆中免疫球蛋白G的含量
%A D Zhou
%A H Zou
%A L Yang
%A L Jia
%A Y Zhang
%A <br>周冬梅
%A 邹汉法
%A 杨利
%A 贾凌云
%A 张玉奎
%J 色谱
%D 1998
%I 
%X An assay technique for determination of Human IgG in human plasma has been developed by utilizing Protein A bound to the modified cellulose matrices based on the strong affinity between protein A and Fc region of IgG. The pH values of loading buffer and elution buffer were 7.0 and 2.3 respectively. The cartridge used was 20 mm x 4 mm i.d. and detection was carried out with a UV monitor at 280 nm. The non-specific adsorption of two kinds of media has been studied. The media without hexyldiamine arm gives very low non-specific adsorption BSA. The calibration curve showed a good linearity (correlation coefficient > 0.9992) in the injected amount range of 9-70 micrograms for HIgG. The total time for separation and determination was within 5 min and the total time of rapid assay was within 30 s. Relative standard deviations of peak areas were 1.5% (n = 5) and 3.6% (n = 3) for HIgG in standard solution and human plasma respectively.
%K NULL<br>高效亲和膜色谱
%K 蛋白A
%K 人免疫球蛋白G
%K HIgG
%K 分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63D957879552F6F07F3&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=38B194292C032A66&sid=64963996248CBF47&eid=2BA123C6EB9D54C2&journal_id=1000-8713&journal_name=色谱&referenced_num=6&reference_num=3