%0 Journal Article
%T Determination of the Azetidine-2-Carboxylic Acid from Lily of the Valley<br>Ò©ÓÃÖ²ÎïÁåÀ¼ÖÐß¹¶¡à¤-2-ôÈËáµÄ²â¶¨
%A Liu Huiwen
%A <br>Áõ»ÝÎÄ
%J É«Æ×
%D 1999
%I 
%X This paper reports the separation and determination of the azetidine-2-carboxylic acid in lily of the valley by high performance liquid chromatography (HPLC) with post-column derivatization and fluorescence detection. The operating conditions were cation exchange resin column (30 cm x 0.4 cm i.d.) with buffer solution A 1.96% (mass fraction) sodium citrate solution pH 3.10 +/- 0.02] and B 2.1% (mass fraction) sodium nitrate solution pH 9.60 +/- 0.02] as mobile phase at flow-rate of 0.4 mL/min, gradient elution, column temperature 65 degrees C, detection wavelength lambda ex = 338 nm and lambda em = 425 nm. The retention time of azetidine-2-carboxylic acid was 5.86 minutes. The recovery was 96.4% and coefficient of variation was 0.94%. This method is simple, rapid and sensitive.
%K high performance liquid chromatography
%K lily of the valley
%K azetidine-2-carboxylic acid<br>ÁåÀ¼
%K ß¹¶¡à¤£­2£­ôÈËá
%K HPLC
%K ß¹¶¡à¤ôÈËá
%K ²â¶¨
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B86253677E873F304A76&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=E158A972A605785F&sid=FED44C0135DC1D9C&eid=216EFB25F7F834CC&journal_id=1000-8713&journal_name=É«Æ×&referenced_num=0&reference_num=2