%0 Journal Article
%T Determination of Angiotensin Converting Enzyme Inhibitor Activity by High Performance Liquid Chromatography<br>高效液相色谱法测定血管紧张素转化酶抑制剂的活性
%A WU Qiongying
%A MA Haile
%A LUO Lin
%A WU Shouyi
%A <br>吴琼英
%A 马海乐
%A 骆琳
%A 吴守一
%J 色谱
%D 2005
%I 
%X A high performance liquid chromatographic method to determine angiotensin-converting enzyme inhibitor activity in vitro was established by using N-hippuryl-His-Leu tetrahydrate as the reaction substrate and hippuric acid as the reaction product. The chromatographic conditions were as follows: column, ZORBAX SB-C18 (4.6 mm i. d. x 150 mm, 5 microm); column temperature, 25 degrees C; mobile phase, acetonitrile-distilled water (25:75, v/v, both containing 0.05% (v/v) trifluoroacetic acid and 0.1% (v/v) triethylamine); flow rate, 0.5 mL/min; detection wavelength, 228 nm. An excellent linearity over the range of 0.005-1.000 mmol/L (r = 0.9999) was observed. The detection limit was 0.50 micromol/L. The recoveries of hippuric acid ware 99.48%-105.64%, with a relative standard deviation (RSD) of 2.20% (n = 6). It is a simple, precise and reliable assay method for developing antihypertension drugs.
%K high performance liquid chromatography
%K angiotensin-converting enzyme inhibitor
%K hippuric acid<br>高效液相色谱法
%K 血管紧张素转化酶抑制剂
%K 马尿酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=78D7849AFECF9CA4&yid=2DD7160C83D0ACED&vid=EA389574707BDED3&iid=CA4FD0336C81A37A&sid=9C65ADEB5990B252&eid=35FC3610259C2B32&journal_id=1000-8713&journal_name=色谱&referenced_num=20&reference_num=7