%0 Journal Article
%T Determination of total sulfite in wine by high performance liquid chromatography(HPLC) with fluorescence detection]<br>高效液相色谱-荧光检测法测定葡萄酒中总亚硫酸盐
%A F Wei
%A M S Chen
%A L Yu
%A L Chang
%A D M Li
%A <br>卫锋
%A 陈明生
%A 于玲
%A 常丽
%A 李冬敏
%J 色谱
%D 1999
%I 
%X A highly sensitive fluorometric method for the determination of total sulfite in wine is reported. The strong fluorescent derivatives formed by the quantitative reaction of sulfite in wine and N-(9-acridinyl) maleimide(NAM) in aqueous solution under mild conditions was injected into the HPLC. Separation was performed on an ODS column(200 mm x 5 mm) with a mobile phase of V(CH3CN):V(0.1 mo/L NH4AC) = 25:75 at a flow rate of 1.0 mL/min and detected by fluorometry at 455 nm(excitation at 365 nm). There was a good linear calibration curve in the range from 0.25-4 mg/L(r = 0.9998), The limit of detection was 1 ng. Sulfite mass concentration in wine has been determined by this method with good results and recoveries. This method is simple, rapid and reliable, and a sample size of only 1 mL is required to complete an analysis.
%K 高效液相色谱
%K 荧光检测
%K 亚硫酸盐
%K 葡萄酒
%K 测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862E0A52252180A8F6E&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=B31275AF3241DB2D&sid=7882A2973AA04DE8&eid=E513158F1BE1471F&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=0