%0 Journal Article
%T Linear scale-up of the separation of active components from Oroxylum indicum using high-speed counter-current chromatography<br>高速逆流色谱在分离纯化木蝴蝶活性成分中的线性放大
%A YUAN Yuan
%A LUO Houding
%A CHEN Lijuan
%A <br>袁媛
%A 骆厚鼎
%A 陈俐娟
%J 色谱
%D 2008
%I 
%X High-speed counter-current chromatography was used to separate and purify flavonoids from the ethyl acetate extract of Oroxylum indicum. After the optimization of separation conditions on analytical instrument, including the two-phase solvent system, rotation speed, flow rate, sample volume and sample concentration, a linear scale-up procedure was performed at preparative grade. Chrysin (160.9 mg, 97.3% in purity), baicalein (130.4 mg, 97.6% in purity), baicalein-7-O-glucoside (314.0 mg, 98.3% in purity), baicalein-7-O-diglucoside (179.1 mg, 99.2% in purity), and a new chrysin-diglucoside (21.7 mg, 98.8% in purity) were obtained from 911.6 mg ethyl acetate extract of Oroxylum indicum by only one step. These five compounds were identified using high performance liquid chromatography, mass spectrometry and nuclear magnetic resonance. With the improvement of the throughput for 53 times after such a scale-up, the resolution and the separation time were kept as the same as those of the analytical grade separation. Therefore, the linear scale-up provided an efficient method for the separation of natural products.
%K high-speed counter-current chromatography (HSCCC)
%K linear scale-up
%K flavonoid
%K Oroxylum indicum
%K Chinese herbal medicine<br>高速逆流色谱
%K 线性放大
%K 黄酮
%K 木蝴蝶
%K 中草药
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=410BEF68EFA5FA6EA7CDF74C6BDC9086&yid=67289AFF6305E306&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=F176272DD933A0BB&eid=8C267C8DC97FEEEF&journal_id=1000-8713&journal_name=色谱&referenced_num=3&reference_num=13