%0 Journal Article
%T Identification of Cell-Surface Molecular Interactions under Living Conditions by Using the Enzyme-Mediated Activation of Radical Sources (EMARS) Method
%A Koichi Honke
%A Norihiro Kotani
%J Sensors
%D 2012
%I MDPI AG
%R 10.3390/s121216037
%X Important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. We have developed a novel method termed enzyme-mediated activation of radical sources (EMARS) to identify coclustering molecules on the cell surface under living conditions, which features a radical formation from an aryl azide reagent by horseradish peroxidase (HRP). For identification of molecules labeled by the EMARS reaction, antibody array system and mass spectrometry-based proteomics approaches are available. Spatio- temporally-regulated interaction between b1 integrin and ErbB4 involved in fibronectin-dependent cell migration and therapeutic antibody-stimulated interaction between FGFR3 and CD20 were discovered using the EMARS method.
%K molecular interaction
%K membrane microdomains
%K lipid rafts
%K EMARS
%K HRP
%K receptor tyrosine kinases
%K antibody array
%K proteomics
%U http://www.mdpi.com/1424-8220/12/12/16037