%0 Journal Article
%T Simultaneous determination of stimulant, narcotics and antiestrogen in urine by gas chromatography-nitrogen phosphorous detection<br>气相色谱-氮磷检测方法同时检测尿样中的刺激剂、麻醉剂和抗雌激素类五种兴奋剂
%A QIU Lijun
%A ZHENG Xiaoyan
%A YOU Feiming
%A LIU Wei
%A ZHANG Jinzhang
%A ZHANG Lan
%A <br>邱丽君
%A 郑小严
%A 游飞明
%A 刘薇
%A 张金章
%A 张兰
%J 色谱
%D 2009
%I 
%X An easy, sensitive and quick method was established for simultaneously separating and determining stimulant, narcotics and antiestrogen in spiked human urine using gas chromatography-nitrogen phosphorous detection (GC-NPD). The urine sample was preprocessed by liquid-liquid extraction. Tert-butyl methyl ether and N-phenylamine were chosen as extraction solvent and internal standard for quantitation, respectively. That is, a standard stock mixture containing methylephedrine, meperidine, methadone, tamoxifen, fentanyl and N-phenylaniline was added into 5.0 mL urine samples and mixed uniformly, then 0.5 mL 5.0 mol/L NaOH, 3.0 g NaCl and 5.0 mL tert-butyl methyl ether were added and finally centrifuged. The extraction solution was dried under N2, redissolved by acetone and then determined by GC-NPD. The method showed the satisfactory linearity was between 0.022~20 mg/L, with the coefficient correlation from 0.9945 to 0.9998. The detection limits were in the range of 0.007~0.015 mg/L, and the average recoveries in spiked urine were between 75.8%~118.2% and the relative standard deviations were lower than 17.2%.
%K gas chromatography-nitrogen phosphorous detection
%K stimulant
%K narcotics
%K antiestrogen
%K doping
%K urine<br>气相色谱-氮磷检测
%K 刺激剂
%K 麻醉剂
%K 抗雌激素
%K 兴奋剂
%K 尿
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=8375E43CC8037BCA0F52CBAA4434D746&yid=DE12191FBD62783C&vid=DB817633AA4F79B9&iid=38B194292C032A66&sid=F7496043B4F12D75&eid=4C2B9916B58305BE&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=15