%0 Journal Article
%T Simultaneous Determination of Aspartame and Amino Acids in Fermented Milk Beverages by HPLC<br>反相高效液相色谱法同时测定发酵乳中的蛋白糖和氨基酸
%A W Zhang
%A X Sun
%A Q She
%A X Zhang
%A <br>张文悦
%A 孙雪雁
%A 佘琼群
%A 张遐耘
%J 色谱
%D 1998
%I 
%X An RP-HPLC method for the simultaneous determination of aspartame and amino acids in fermented milk beverages were established. Samples were prepared by mixing with methanol at a ratio of 1:1 and centrifuged at 4,000 r/min for 15 min. Ten microL supernatant was moved into a sample tube, dried and derivatized according to PICO-TAG procedure developed by Waters. A Novapak C18 column (3.9 mm x 150 mm) was used instead of PICO TAG column and the gradient elution program was modified correspondingly. Column temperature was maintained at 38 degrees C and the components were detected at 254 nm. Linearity for aspartame at the range in 1-100 mg/L is A = 333 C + 20 with r = 0.9996 where A is the integrated area of chromatographic peak of aspartame and C is the corresponding mass concentration. Repeatability for 8 injections was tested and the RSD of 3.2% was obtained. The recovery of aspartame for 5 samples with the method ranged from 94.2% to 98.7%.
%K reversed
%K phase high performance liquid chromatography
%K PICO
%K TAG system
%K aspartame
%K amino acids<br>HPLC
%K 蛋白糖
%K 氨基酸
%K 发酵乳制品
%K 分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63D9E5625632CE315E8&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=B31275AF3241DB2D&sid=92DA076AF6760FAC&eid=DFBC046213B3DD86&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=0