%0 Journal Article
%T Study of the Acupuncture Effect on Monoamine Transmitters in Rabbit Plasma and Brain Tissue by High Performance Liquid Chromatography with Electrochemical Detection<br>高效液相-电化学检测法研究针刺对家兔血浆及脑组织中单胺类神经递质的影响
%A HOU Jing-guo
%A LIU Hui-ling
%A HE Tian-xi
%A WANG Zhu-ming
%A MAO Xue-feng
%A DU Xin-zhen
%A DENG Hua-ling
%A GAO Jin-zhang
%A <br>侯经国
%A 刘慧玲
%A 何天稀
%A 王柱命
%A 毛学峰
%A 杜新贞
%A 邓华陵
%A 高锦章
%J 色谱
%D 2002
%I 
%X A rapid and simple method for the study of the acupuncture effect on monoamine transmitters and related compounds in rabbit plasma and brain tissue by high performance liquid chromatography with electrochemical detection was developed. An ODS column was selected as the separation column at 25 degrees C, and pH 4.50, 0.02 mol/L of trisodium citrate-0.05 mol/L sodium phosphate dibasic to methanol (95:5, volume ratio) without ion-pair at a flow rate of 1.0 mL/min. Four compounds, epinephrine (E), norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT), were simultaneously separated and determined under the above conditions. Twenty rabbits were investigated after the acupuncture action upon the central neurotransmitters. The sufficient data showed that acupuncture could significantly affect the activities of the neurotransmitters including E, NE, DA and 5-HT, and the changed functions of the neurotransmitter systems induced by acupuncture not only lead to the neurotransmitter content increase both in brain and plasma but also cause the increase of rabbit breed ability. The results show that the method is very simple and fast. The method is valuable not only for clinical diagnosis but also for research work.
%K high performance liquid chromatography
%K electrochemistry detection
%K monoamine transmitter
%K acupuncture<br>高效液相色谱
%K 电化学检测
%K 针刺
%K 单胺类神经递质
%K 生理功能
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0D47A674B747C7F&yid=C3ACC247184A22C1&vid=A04140E723CB732E&iid=0B39A22176CE99FB&sid=B0EBA60720995721&eid=475189FCB44F11F6&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=9