%0 Journal Article
%T Determination of Aflatoxins in Peanut by High Performance Liquid Chromatograp hy Using Immunoaffinity Column Clean-Up and On-Line Electrochemical Deriva tization<br>免疫亲和柱净化、在线电化学衍生化高效液相色谱法检测花生中的黄曲霉毒素
%A ZHANG Peng
%A ZHANG Yi-bing
%A ZHAO Wei-dong
%A LI Yin-bin
%A <br>张鹏
%A 张艺兵
%A 赵卫东
%A 李寅宾
%J 色谱
%D 2000
%I 
%X A method for the determination of aflatoxin B1, B2, G1 and G2 in peanut by high performance liquid chromatography using immunoaffinity column clean-up and on-line electrochemical derivatization was developed. Sample was extracted with 80% methanol and the extract was passed through the IAC. Aflatoxins were retained by the monoclonal in the IAC and eluted with actonitrile. AFT B1 and G1 were derivatized with on-line electrochemical equipment Kobra Cell. The four toxins can be separated in 13 minutes and the detection limit was 0.1 microgram/kg for each toxin. The method has been applied to the determination of peanut sample. The relative standard deviation was 9.2%-15% and the average recovery of AFT spiked at 0.5-9.0 micrograms/kg levels was in the range of 74.8%-97.3%.
%K high performance liquid chromatography
%K electrochemical derivatization
%K immunoaffinity column
%K aflatoxin
%K peanut<br>高效液相色谱法
%K 电化学衍生
%K 黄曲霉毒素
%K 花生
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=C8AE6339CB75AFC9&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&sid=0D0D661F0B316AD5&eid=656F8C8401D91023&journal_id=1000-8713&journal_name=色谱&referenced_num=10&reference_num=2