%0 Journal Article
%T Application of online two-dimensional separation system using monolithic columns for proteome analysis of human cartilage<br>毛细管整体柱在线二维分离系统应用于人体软骨的蛋白质组分析
%A XIE Shajie
%A WANG Fangjun
%A YAN Dan
%A ZHOU Guangdong
%A YE Mingliang
%A ZOU Hanfa
%A <br>谢沙洁
%A 王方军
%A 晏丹
%A 周广东
%A 叶明亮
%A 邹汉法
%J 色谱
%D 2010
%I 
%X In Shotgun proteome analysis,where nano-flow is adopted to increase the sensitivity as well as extremely complicated samples such as proteolytic digest are inevitably confronted,monolithic capillary columns are widely used to improve the liquid chromatography separation performance.It is known that cartilage contains extensive amounts of extracellular matrix(ECM),in which collagens and aggrecans being the most abundant macromolecules.It is obvious that the high content of ECM components causes a challenge i...
%K monolithic column
%K online two-dimensional separation
%K proteomics
%K cartilage tissue<br>整体柱
%K 在线二维分离
%K 蛋白质组学
%K 软骨组织
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=E41EBB59B3D57C52BDAD33736F3D332A&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=0B39A22176CE99FB&sid=B0EBA60720995721&eid=769BD58726D66E7D&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=27