%0 Journal Article
%T Chromatographic Behavior and Purification of Synthetic Huwentoxin-<br>化学合成虎纹捕鸟蛛毒素-Ⅰ的色谱行为及分离纯化
%A WANG Xian chun
%A LIANG Song ping
%A <br>王贤纯
%A 梁宋平
%J 色谱
%D 2001
%I 
%X A comparative study was made to investigate the chromatographic behavior and purification of chemically synthesized and native polypeptide neurotoxin huwentoxin-I (HWTX-I) by means of reversed-phase HPLC and ion-exchange chromatography. The results showed that the synthetic HWTX-I in crude product has a longer retention time than the native HWTX-I, but has the same retention time as the denatured HWTX-I, suggesting it being in a state of complete denaturation which was confirmed by the MALDI-TOF MS analysis. It has also been discovered that, before complete purification, the synthetic HWTX-I, after oxidative treatment, showed wider and more asymmetric peaks than the native HWTX-I that was denatured and then renatured under the same experimental conditions, which suggests that there is even greater conformational unhomogeneity in the synthetic HWTX-I. It was inferred that, in addition to the wrong disulfide bonds formation during oxidative folding, the racemization during chemical synthesis resulted in more stereoisomers of synthetic HWTX-I. It was needed for the renatured synthetic peptides to be purified using different methods alternatively.
%K chromatography
%K purification
%K polypeptide
%K solid phase synthesis<br>色谱
%K 纯化
%K 固相合成
%K 分离
%K 虎纹捕岛蛛毒素－Ⅰ
%K 神经毒素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=9AC783D97B6E4A35&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=0B39A22176CE99FB&sid=74011071555EB4E5&eid=DBF54A8E2A721A6D&journal_id=1000-8713&journal_name=色谱&referenced_num=3&reference_num=9