%0 Journal Article
%T Determination of Azathiopurine and 6 Mercaptopurine in Serum by Reversed Phase High Performance Liquid Chromatography<br>高效液相色谱法分析人血清中硫唑嘌呤和巯嘌呤的浓度
%A Tang Jianlin
%A Zhou Shiwen
%A Huang Linqing
%A Huang Yongping
%A <br>汤建林
%A 周世文
%A 黄林清
%A 黄永平
%A 张惠静
%J 色谱
%D 1998
%I 
%X A method for simultaneous determination of azathiopurine(AZP) and its metabolite 6-mercaptopurine (6-MP) concentration in serum has been developed. Reversed-phase high performance liquid chromatography with gradient elution and dual wavelength detection was employed. After protein precipitation with acetonitrile, the serum sample was evaporated to dryness with a gentle N2 stream at 37 degrees C and 100 microL of the mobile phase were added to dissolve the residue. After centrifugation, 20 microL of the supernatant was injected directly into the HPLC. The detection wavelength was set at 278 nm (AZP) and 325 nm (6-MP). The internal standard was metronidazole. The minimum detectable mass concentration for both AZP and 6-MP in serum was 5 mg/L. The average recoveries were (100.6 +/- 4.2)% for AZP and (102.4 +/- 4.5)% for 6-MP.
%K high performance liquid chromatography
%K azathiopurine
%K serum drug concentration<br>硫唑嘌呤
%K 巯嘌呤
%K 血药浓度
%K HPLC
%K 免疫抑制剂
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63DB2C3A66D4E29D35B&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=B31275AF3241DB2D&sid=F9A6B6F259CE5121&eid=F204392B3B11C3BD&journal_id=1000-8713&journal_name=色谱&referenced_num=3&reference_num=0