%0 Journal Article
%T Determination of vitamin D3 in health-care foods fortified with calcium by reversed-phase high performance liquid chromatography]<br>反相高效液相色谱法测定补钙保健品中的维生素D_3
%A H Wu
%A G Tang
%A <br>吴红京
%A 唐根源
%J 色谱
%D 1998
%I 
%X "Bu Gai Bao" and "Gaoxiao Man Gai" are health-care foods fortified with calcium for people of all ages. This paper describes a method of reversed-phase high performance liquid chromatography for quantitating vitamin D3 in these products. The method is simple, sensitive, and suitable for routine analysis of vitamin D3 in a variety of health-care foods fortified with calcium. Shimadzu LC-4A high performance liquid chromatograph with SPD-2AS UV detector and C-R2AX data processor were used. The vitamin D3 in products was extracted with chloroform and separated with mu-Bondapak C18 column(300 mm x 3.9 mm i.d.) and methanol water(87:13, V/V) mobile phase with detection at UV-264 nm. Regression coefficient of correlation of calibration curves in the range of 10-50 mg/L was 0.9998. Average recoveries of vitamin D3 added to the products was 95.63%. The coefficients of variation of vitamin D3 in both products were 0.24%-0.49%.
%K NULL<br>HPLC
%K 维生素D3
%K 保健品
%K 补钙保健品
%K 测定
%K 分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63D560BD8A939054195&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=38B194292C032A66&sid=6826CBE9C80ACB20&eid=7979125BBE749348&journal_id=1000-8713&journal_name=色谱&referenced_num=3&reference_num=4