%0 Journal Article
%T Purification of Recombinant Erythropoietin by Reversed Phase High Performance Liquid Chromatography(RP HPLC)<br>反相高效液相色谱法分离重组促红细胞生成素
%A Zou Zhongcheng
%A Sun Kailai
%A Lou Dan
%A Hu Ming
%A <br>邹钟诚
%A 孙开来
%A 娄丹
%A 胡明
%J 色谱
%D 1998
%I 
%X Recombinant erythropoietin is a glycoprotein which is strongly hydrophobic. We purified recombinant erythropoietin by RP-HPLC. The results showed that 30 nm Spherisorb C4 reversed-phase column and acetonitrile-TFA mobile phase with gradient elution, could readily isolate recombinant erythropoietin from crude samples with high speed and efficiency. Erythropoietin could be eluted at the concentration of about 50% acetonitrile with appropriate sample purity and elution gradient. When RP-HPLC is used as a final step of purification, the purity of sample should be at least 60%. The purity of the product tested by SDS-PAGE was nearly 100%. And the specific activity of the product was about 1.96 x 10(3) IU/g protein.
%K reversed phase high performance liquid chromatography
%K erythropoietin
%K purification<br>促红细胞生成素
%K 纯化
%K 分离
%K 重组EPO
%K HPLC
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63D7D47497734A0EFE0&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=38B194292C032A66&sid=96A53C367B5173D7&eid=7ABC4505E3960D2B&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=1