%0 Journal Article
%T Optimization of two-dimensional high performance liquid chromatographic columns for highly efficient separation of intact proteins<br>蛋白质高效分离两维色谱柱的选择优化
%A HONG Guangfeng
%A GAO Mingxia
%A YAN Guoquan
%A GUAN Xia
%A TAO Qian
%A ZHANG Xiangmin
%A <br>洪广峰
%A 高明霞
%A 晏国全
%A 关霞
%A 陶芊
%A 张祥民
%J 色谱
%D 2010
%I 
%X In order to optimize two-dimensional liquid chromatographic(2D-LC) columns for highly efficient separation of proteins,several liquid chromatographic columns were investigated and evaluated.Weak anion-exchange(WAX) column was chosen as the first dimension because of its extensive protein separation power.By comparison of different WAX chromatographic columns for human liver protein separation,TSKgel DEAE-5PW column was selected as the first dimension of a 2D-LC system.For the second dimension,ten typical re...
%K proteomics
%K multidimensional liquid chromatography
%K intact protein
%K Top-down<br>蛋白质组学
%K 多维液相色谱
%K 蛋白质
%K Top-down技术
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=C1F062F6EA57D6C5BF0177295D04E48E&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=0B39A22176CE99FB&sid=4C100B7696CE9E24&eid=F1177A9DF1349B63&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=17