%0 Journal Article
%T Determination of 4-Aminobutyric Acid in Pumpkin Powderby High Performance Liquid Chromatography<br>高效液相色谱法测定南瓜粉中的4-氨基丁酸
%A LIU Hui-wen
%A <br>刘惠文
%J 色谱
%D 2001
%I 
%X A method for the separation and determination of the 4-aminobutyric acid in pumpkin powder by high performance liquid chromatography (HPLC) with post-column derivatization and fluorescence detection is described. The operating conditions were cation exchange resin column (30 cm x 0.4 cm i.d.) with gradient elution of buffer solutions A 19.6 g sodium citrate dihydrate and 1.0 g phenol dissolved in 1 L water, pH(3.15 +/- 0.02)] and B 21.0 g sodium nitrate and 1.5 g boric acid dissolved in 1 L water, pH(9.70 +/- 0.02)] as mobile phase at a flow rate of 0.5 mL/min, and a column temperature of 62 degrees C, with detection wavelength lambda ex = 338 nm, lambda em = 425 nm. The retention time of 4-aminobutyric acid was 34.77 minutes. The average recovery was 99% and the coefficient of variation was 1.35%. This method is simple, rapid and sensitive.
%K high performance liquid chromatography
%K post
%K column derivatization
%K 4
%K aminobutyric acid<br>高效液相色谱法
%K 柱后衍生
%K 4－氨基丁酸
%K 南瓜粉
%K 强离子交换柱
%K 分离
%K 测定
%K 分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=10FBDDAB7EED5726&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=B31275AF3241DB2D&sid=640CCB6E396307A8&eid=FF58680609C9D068&journal_id=1000-8713&journal_name=色谱&referenced_num=14&reference_num=4