%0 Journal Article
%T Determination of Glycyrrhizinic Acid in Biotransformation System by Reversed-Phase High Performance Liquid Chromatography<br>反相高效液相色谱法测定生物转化体系中的甘草酸
%A LI Hui~
%A LU Dingqiang~
%A LIU Weimin~
%A <br>李晖
%A 卢定强
%A 刘伟民
%J 色谱
%D 2004
%I 
%X A method for determining glycyrrhizinic acid in the biotransformation system by reversed-phase high performance liquid chromatography (RP-HPLC) was developed. The HPLC conditions were as follows: Hypersil C18 column (4.6 mm i.d. x 250 mm, 5 microm) with a mixture of methanol-water-acetic acid (70:30:1, v/v) as the mobile phase; flow rate at 1.0 mL/min; and UV detection at 254 nm. The linear range of glycyrrhizinic acid was 0.2-20 microg. The recoveries were 98%-103% with relative standard deviations between 0.16% and 1.58% (n = 3). The method is simple, rapid and accurate for determining glycyrrhizinic acid.
%K high performance liquid chromatography
%K glycyrrhizinic acid
%K biotransformation system<br>高效液相色谱法
%K 甘草酸
%K 生物（酶）转化体系
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=DD6EFC448C70E878&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=38B194292C032A66&sid=B799C1769FCACDC8&eid=0F7768518993EDDE&journal_id=1000-8713&journal_name=色谱&referenced_num=6&reference_num=8