%0 Journal Article
%T Separation of Levonorgestrel, Progesterone and Testosterone by Micellar Electrokinetic Capillary Chromatography<br>胶束电动毛细管色谱法分离左旋十八甲基炔诺酮、睾酮和孕酮
%A Xu Qijin
%A Zhang Ying
%A Gu Zhongwei
%A Chen Xianli
%A <br>徐其进
%A 张英
%A 顾忠伟
%A 陈先丽
%J 色谱
%D 1999
%I 
%X Separation of levonorgestrel, progesterone and testosterone was achieved by using micellar electrokinetic capillary chromatography (MECC). A column of 53 cm (to the detector) x 50 microns i.d. uncoated fused-silica capillary (totally 68 cm in length) and an ultra-violet detector with fixed wavelength at 254 nm were used throughout all the experiments. MECC analysis was optimized by evaluating three different micelle-forming agents, the concentration of SDS and several of organic additives in a 10 mmol/L borate running buffer (pH 9.2). Complete separations were obtained with either 10%-20% acetonitrile or 20 mmol/L 2,6-dimethyl-beta-cyclodextrin (DM-beta-CD) as the modifier in buffer. When the acetonitrile volume fraction was in the range of 0-15%, the migration times of the three steroids increased with the acetonitrile volume fraction, but in the range of 15%-20% acetonitrile concentrations, the situation was opposite. This behavior of the steroids was attributed to the interaction of two opposite effects, an increased mobility due to decreased partition coefficient and a decreased electroosmotic flow (EOF). Both beta-cyclodextrin (beta-CD) and gamma-cyclodextrin (gamma-CD) were found to be inadequate for a satisfactory separation.
%K micellar electrokinetic capillary chromatography
%K levonorgestrel
%K steroid hormones<br>胶束电动毛细管色谱法
%K 左旋十八甲基炔诺酮
%K 甾体激素
%K 分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862BF43AA9DD1A2AD86&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=3E0812ED84A7B31D&eid=3A0155B37D8FF829&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=2