%0 Journal Article
%T Simultaneous Determination of Four Carotenoids in Health Products by Reversed-Phase High Performance Liquid Chromatography<br>反相高效液相色谱法同时测定保健品中的四种类胡萝卜素
%A LI He
%A CHEN Min
%A ZHU Lei
%A LIU Lijuan
%A WANG Jingyu
%A <br>李赫
%A 陈敏
%A 朱蕾
%A 刘丽娟
%A 王静钰
%J 色谱
%D 2006
%I 
%X A method was established for the determination of carotenoids (lutein, zeaxanthin, lycopene, beta-carotene) in health products by reversed-phase high performance liquid chromatography (RP-HPLC). The extraction methods of carotenoids from different types of samples were also established. Separation and quantification was achieved by using a Diamonsil C18 column (250 mm x 4.6 mm i.d., 5 microm), and a mobile phase of acetonitrile and ethyl acetate were used with the following gradient elution: 100% acetonitrile at the first 15 min, then ethyl acetate increased to 100% in the next 10 min, at last 100% acetonitrile for 5 min with a flow rate of 1 mL/min, temperature of 25 degrees C and monitored with a photodiode array detector at 450 nm. The external standard calibration curves were used for quantification. There were good linear correlations for the four carotenoids. The correlation coefficients were between 0.999 4 and 0. 999 7. The detection limits were 0.4-0.5 mg/L. The recoveries of four carotenoids in tablet samples were 95.3%-98.7% with the relative standard deviations (RSDs) of 0.89%-2.0%. The recoveries of four carotenoids in powder samples were 93.7%-98.8% with RSDs of 0.89%-2.8%. The recoveries of four carotenoids in oil samples were 97.1%-99.2% with RSDs of 0.42%-1.2%. This method is simple, fast, accurate and convenient. It can be used as one of the reliable means for quantitative determination of carotenoid in health products.
%K 反相高效液相色谱
%K 类胡萝卜素
%K 番茄红素
%K 叶黄素
%K 玉米黄素
%K β-胡萝卜素
%K 保健品
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=B97D457FDD2CFE54&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=D93AD940782892D0&eid=A94616C4391AA5EF&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=11