%0 Journal Article
%T Rapid Determination of Aspartam in Compound Sweetening by Reversed Phase High Performance Liquid Chromatography<br>反相高效液相色谱法快速测定复合甜味剂中Aspartam
%A R Zhang
%A M Jiang
%A <br>章日春
%A 蒋明哲
%J 色谱
%D 1997
%I 
%X A method for rapid determination of Aspartame in compound sweetening by reversed-phase high performance liquid chromatography is presented. Aspartame in compound sweetening was separated in a short column (Ultrasphere XL-ODS, 3 microm, 4.6 mm x 70 mm) by using CH3OH-0.02 mol/L NH4Ac as mobile phase. The flow rate was 0.8 mL/min. Detection was performed with UV detector at 220 nm. The injection volume was 20 microL. It was qualitatively analysed by UV scanning at a wavelength range of 200-350 nm under no-stop flow according to their retention time. Quantitative analysis was carried out by measuring peak height and comparing it with external standard. The minimum detectable amount was 5 microg/L. The linear range of the calibration curve was 40-200 mg/L. The average recovery of Aspartame was 92%. The relative standard deviation was 2.9%. This method is simple, rapid and sensitive.
%K reversed phase high performance liquid chromatography
%K UV scanning
%K Aspartam
%K compound sweetening<br>HPLC
%K 甜味素
%K 复合甜味剂
%K Aspartam
%K 天苯甜
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0C7CB2C65AD7C6DF140FE8397267A8D&yid=5370399DC954B911&vid=23CCDDCD68FFCC2F&iid=B31275AF3241DB2D&sid=8C044EC256B1039D&eid=BA305A52E2EE9350&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=1