%0 Journal Article
%T Determination of Berberine in Human Serum by Reversed Phase High Performance Liquid Chromatography<br>RP-HPLC测定人血清中黄连素浓度
%A H Zhang
%A Z Shao
%A Y Sun
%A <br>张宏文
%A 邵志高
%A 孙一勤
%J 色谱
%D 1997
%I 
%X Berberine is a safe and effective anti-arrhythmic drug. We do not clearly know about the dose-effect relationship. Besides, berberine is not fit for administration by i.m. and i.v.. Therefore, it is necessary to build a PD model of berberine, study its pharmacodynamics parameters in vivo, and provide a concrete criteria in the clinical usage. In this article, we set up a method for determination of concentration of berberine in human serum by RP-HPLC with Lichrosorb RP-18 column (250 mm x 4 mm) and mobile phase of MeOH containing 0.15% triethylamine. The content of berberine was determined by the external standard method with UV detector at 347 nm. The linear range was 0.2-2 mg/L (r = 0.9996). The detectable limit was 2.55 ng. The average recovery was 90.73% and the coefficients of variation were below 8.0%. The method is sensitive and accurate.
%K high performance liquid chromatography
%K berberine
%K drug levels in serum<br>高效液相色谱
%K 黄连素
%K 血药浓度
%K 人血清
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0C7CB2C65AD7C6D7CE56696C736FEE0&yid=5370399DC954B911&vid=23CCDDCD68FFCC2F&iid=94C357A881DFC066&sid=B1989ED92BA7E896&eid=366B1248A15658C5&journal_id=1000-8713&journal_name=色谱&referenced_num=3&reference_num=3